Enzymes of the Poly-β-Hydroxybutyrate and Citric Acid Cycles of Rhizobium japonicum Bacteroids
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Enzymes of the Poly-beta-Hydroxybutyrate and Citric Acid Cycles of Rhizobium japonicum Bacteroids.
The activities of several enzymes of the citric acid and poly-beta-hydroxybutyrate cycles were measured in Rhizobium japonicum 3I1B-143 bacteroids which had been isolated from soybean nodules by sucrose gradient centrifugation. During the period of developing nitrogenase activity, the specific activity of fumarase, hydroxybutyrate dehydrogenase, beta-ketothiolase, and pyruvate dehydrogenase com...
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Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-beta-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. T...
متن کاملOn the DNA Content of the Bacteroids of Rhizobium japonicum
DNA Content, Nodules, Bacteroids, Rhizobium, Soybean The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those...
متن کاملCarbon Metabolism Enzymes of Rhizobium tropici Cultures and Bacteroids.
We determined the activities of selected enzymes involved in carbon metabolism in free-living cells of Rhizobium tropici CFN299 grown in minimal medium with different carbon sources and in bacteroids of the same strain. The set of enzymatic activities in sucrose-grown cells suggests that the pentose phosphate pathway, with the participation of the Entner-Doudoroff pathway, is probably the prima...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1984
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.75.4.1158